3MDF
Crystal structure of the RRM domain of Cyclophilin 33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | NOIR1 |
Collection date | 2008-04-22 |
Detector | MBC DETECTOR |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.840, 40.520, 65.660 |
Unit cell angles | 90.00, 127.06, 90.00 |
Refinement procedure
Resolution | 26.200 - 1.850 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cvj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | BALBES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.200 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.075 | 0.232 |
Number of reflections | 15587 | |
<I/σ(I)> | 15.6 | 5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.76 | 6.41 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 291 | 0.1 M Hepes and 1.0 M tri-sodium dehydrate citrate pH 7.6, Microcapillary, temperature 291K |