3MD3
Crystal Structure of the First Two RRM Domains of Yeast Poly(U) Binding Protein (Pub1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-22 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.615, 57.615, 237.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.900 - 2.700 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.939 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.117 | 0.068 | 0.324 |
| Number of reflections | 7092 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 99.3 | 95.7 | 99.7 |
| Redundancy | 6 | 5.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 283 | 2.0M ammonium sulfate, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
