3MCB

Crystal structure of NAC domains of human nascent polypeptide-associated complex (NAC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	59.794, 59.794, 156.763
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.368 - 1.900
R-factor	0.194
Rwork	0.188
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.135
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.051	0.032	0.533
Number of reflections	13682
<I/σ(I)>	14.7
Completeness [%]	98.8	94.7	94.9
Redundancy	18.3	18	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	289	0.1M sodium citrate (pH 5.6), 0.1 M NaCl, 12% (w/v) PEG 4000, VAPOR DIFFUSION, temperature 289K