3MBX
Crystal structure of chimeric antibody X836
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 118 |
| Detector technology | CCD |
| Collection date | 2007-06-28 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.930, 99.840, 125.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.21062 |
| Rwork | 0.210 |
| R-free | 0.23204 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Q9Q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.411 |
| Data reduction software | d*TREK (9.6L) |
| Data scaling software | d*TREK (9.6L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.088 | 0.436 |
| Number of reflections | 69807 | |
| <I/σ(I)> | 12.6 | 3 |
| Completeness [%] | 96.9 | 91.8 |
| Redundancy | 8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1 M PHOSPHATE-CITRATE PH 4.2, 0.2 M SODIUM CHLORIDE, 10% PEG 3350; CRYO CONDITIONS: MOTHER LIQUOR + 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
