3MBP

MALTODEXTRIN-BINDING PROTEIN WITH BOUND MALTOTRIOSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1989-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	C 1 2 1
Unit cell lengths	106.070, 68.430, 57.940
Unit cell angles	90.00, 112.51, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.164 *
Rwork	0.164
Structure solution method	ISOMORPHOUS WITH 2MBP
Starting model (for MR)	2MBP
RMSD bond length	0.027
RMSD bond angle	0.053
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Data scaling software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	PROTEIN
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.820
High resolution limit [Å]	1.700	1.700
Rmerge	0.065	0.180
Number of reflections	39297
<I/σ(I)>	13.88	2.5
Completeness [%]	86.0	46
Redundancy	3.7	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2	4 *	PROTEIN WAS CRYSTALLIZED FROM 18% PEG 8000, 10 MM CITRATE, PH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MBP	3.5 (mg/ml)
2	1	drop	maltodextrin	1 (mM)
3	1	drop	PEG6000	16 (%(w/v))
4	1	reservoir	PEG6000	22 (%(w/v))