3MBD
Crystal structure of fructose bisphosphate aldolase from Encephalitozoon cuniculi, bound to phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9774 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.960, 137.610, 62.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.340 - 2.000 |
R-factor | 0.16375 |
Rwork | 0.162 |
R-free | 0.19021 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qo5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.395 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.800 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.489 |
Number of reflections | 35720 | |
<I/σ(I)> | 21.83 | 3.6 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 6.2 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 90% PACT SCREEN CONDITION F10, 10% ADDITIVE SCREEN G12: 90MM BIS-TRIS PROPANE PH 6.5, 18% PEG 3350, 18 MM NAKHPO4, 10 MM UREA, PROTEIN AT 24.7 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |