3MBB
Crystal structure of StSPL - apo form, after treatment with semicarbazide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-06-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.650, 126.990, 136.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.410 - 2.051 |
R-factor | 0.1816 |
Rwork | 0.181 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Non-deposited but refined structure of dimeric StSPL K311A |
RMSD bond length | 0.007 |
RMSD bond angle | 1.088 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 63157 | |
<I/σ(I)> | 16.4 | 4.5 |
Completeness [%] | 98.9 | 95 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | PEG 5000 MME 17%, 0.15 M KSCN, 0.1 M Tris(HOAc) pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |