3MAN
MANNOHEXAOSE COMPLEX OF THERMOMONOSPORA FUSCA BETA-MANNANASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.869, 71.729, 98.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.600 |
R-factor | 0.128 * |
Rwork | 0.128 |
R-free | 0.16600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2man |
RMSD bond length | 0.009 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.062 | 0.472 |
Number of reflections | 37351 | |
<I/σ(I)> | 18 | 2.6 |
Completeness [%] | 83.9 | 84.5 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 1.85M AMMONIUM SULFATE, 0.1M HEPES, PH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.85 (M) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |