3MAM
A molecular switch changes the low to the high affinity state in the substrate binding protein AfProX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 73 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.792, 36.958, 57.886 |
| Unit cell angles | 96.07, 99.57, 96.12 |
Refinement procedure
| Resolution | 19.990 - 1.800 |
| R-factor | 0.20407 |
| Rwork | 0.202 |
| R-free | 0.24681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sw5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.078 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.114 |
| Number of reflections | 22833 | |
| <I/σ(I)> | 27.9 | 12.08 |
| Completeness [%] | 95.0 | 95.1 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM HEPES, 15-25% PEG6000, 15-25% PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
