3MAL
Crystal structure of the SDF2-like protein from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 |
Unit cell lengths | 96.130, 96.130, 69.343 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.300 - 1.950 |
R-factor | 0.16921 |
Rwork | 0.167 |
R-free | 0.21279 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t9f |
RMSD bond length | 0.013 |
RMSD bond angle | 1.506 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.300 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.116 | 0.380 |
Number of reflections | 26693 | |
<I/σ(I)> | 14 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 0.1 M Hepes, 0.2 M ammonium sulphate, 22.5% (w/v) PEG 3350, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |