3MAH
A putative c-terminal regulatory domain of aspartate kinase from porphyromonas gingivalis w83.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 46.670, 46.670, 221.902 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.420 - 2.310 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.721 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.420 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.194 |
| Number of reflections | 6972 | |
| <I/σ(I)> | 63.141 | 18.4 |
| Completeness [%] | 98.7 | 98.4 |
| Redundancy | 15.2 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.1 M Tris, 1.6 M Ammonium Sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
