3MA9
Crystal structure of gp41 derived protein complexed with fab 8066
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-03 |
Detector | marccd 300 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.527, 124.698, 133.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.450 - 2.050 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.24512 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cmr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.209 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 44497 | |
<I/σ(I)> | 15.24 | 1.92 |
Completeness [%] | 99.2 | 96.6 |
Redundancy | 4.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.2M dibasic Ammonium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |