3MA5

Crystal structure of the tetratricopeptide repeat domain protein Q2S6C5_SALRD from Salinibacter ruber. Northeast Structural Genomics Consortium Target SrR115c.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Detector technology	CCD
Collection date	2010-03-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.97869
Spacegroup name	P 65 2 2
Unit cell lengths	84.360, 84.360, 227.547
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.885 - 2.800
R-factor	0.2408
Rwork	0.239
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kcl
RMSD bond length	0.009
RMSD bond angle	1.488
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.076	0.941
Number of reflections	22372
<I/σ(I)>	59.1	3.47
Completeness [%]	99.9	100
Redundancy	21.5	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under Paraffin oil	7	291	45-60% PEG 400, 0.03-0.05M potassium chloride, 0.1M Bis-Tris, pH 7.0, microbatch under Paraffin oil, temperature 291K