3M9X
Open liganded crystal structure of xylose binding protein from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.718, 71.706, 66.356 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.19979 |
| Rwork | 0.197 |
| R-free | 0.25573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m9w |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.363 | |
| Number of reflections | 14880 | |
| <I/σ(I)> | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 21%(w/v) polyethylene glycol 3350, 0.2M ammonium dihydrogen phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
