3M9U
Crystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-03-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 48.496, 51.096, 125.928 |
Unit cell angles | 95.47, 91.28, 105.56 |
Refinement procedure
Resolution | 20.000 - 1.770 |
R-factor | 0.15933 |
Rwork | 0.158 |
R-free | 0.19626 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.370 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.680 | |
Number of reflections | 113051 | |
<I/σ(I)> | 3.4 | 1.3 |
Completeness [%] | 95.6 | 93.8 |
Redundancy | 1.3 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 0.1M SODIUM ACETATE, PH 4.5, 25% PEG3350, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |