3M9S
Crystal structure of respiratory complex I from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.390, 336.090, 262.240 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3I9V AND 3M9C |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.740 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.252 | 0.628 |
| Number of reflections | 72549 | |
| <I/σ(I)> | 2.5 | 1 |
| Completeness [%] | 74.9 | 65.1 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
