3M9C
Crystal structure of the membrane domain of respiratory complex I from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.060, 182.170, 185.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.900 |
| Structure solution method | MIRAS |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.205 | 0.846 |
| Number of reflections | 34983 | |
| <I/σ(I)> | 4 | 1.5 |
| Completeness [%] | 97.6 | 97.4 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 283 | 9% PEG4000, 0.1 M sodium acetate, 1.0 M sodium formate, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
