3M9B
Crystal structure of the amino terminal coiled coil domain and the inter domain of the Mycobacterium tuberculosis proteasomal ATPase Mpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 176.787, 176.652, 176.633 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.940 |
| R-factor | 0.2762 |
| Rwork | 0.275 |
| R-free | 0.30369 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.234 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 4.010 |
| High resolution limit [Å] | 3.940 | 3.940 |
| Rmerge | 0.046 | 0.510 |
| Number of reflections | 98265 | |
| Completeness [%] | 99.8 | 60 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30%v/v Tacsimate (1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
