3M8K
Protein structure of type III plasmid segregation TubZ
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97963,1.01995,0.97974 |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.601, 131.601, 76.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.790 - 2.300 |
| R-factor | 0.21542 |
| Rwork | 0.214 |
| R-free | 0.25077 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.790 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.271 |
| Number of reflections | 21740 | |
| <I/σ(I)> | 19.4 | 4.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4 M NaFormate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
