3M8J
Crystal structure of E.coli FocB at 1.4 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792, 0.97945, 0.97535 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.390, 58.823, 65.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.790 - 1.400 |
| R-factor | 0.19745 |
| Rwork | 0.196 |
| R-free | 0.22160 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.611 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.036 | 0.314 |
| Number of reflections | 36163 | |
| <I/σ(I)> | 28.6 | 6.1 |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1.5M magnesium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
