3M8F
Protein structure of type III plasmid segregation TubR mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-15 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.400, 79.518, 32.351 |
| Unit cell angles | 90.00, 102.06, 90.00 |
Refinement procedure
| Resolution | 31.370 - 2.800 |
| R-factor | 0.20696 |
| Rwork | 0.204 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m8e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.224 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.045 | 0.382 |
| Number of reflections | 5671 | |
| <I/σ(I)> | 22.5 | 2.6 |
| Completeness [%] | 88.5 | 86.4 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 100 mM HEPES pH 7.5, 8% ethylene glycol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
