3M8E
Protein structure of Type III plasmid segregation TubR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95704,0.97966 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.499, 61.556, 51.249 |
| Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
| Resolution | 49.810 - 2.000 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.810 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.267 |
| Number of reflections | 17963 | |
| <I/σ(I)> | 21.9 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 293 | 40 mM Na/K phosphate, 2.5 M NaCl, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
