3M8A
Crystal Structure of Swine Flu Virus NS1 N-Terminal RNA Binding Domain from H1N1 Influenza A/California/07/2009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.847, 156.795, 73.523 |
| Unit cell angles | 90.00, 109.21, 90.00 |
Refinement procedure
| Resolution | 29.250 - 2.100 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z0a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.094 | 0.511 |
| Number of reflections | 75867 | |
| <I/σ(I)> | 18.9 | 3.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tacsimate 35%, ph 7, KCl 0.1M, MgCl2 0.1M, 0.3M NDSB211, TCEP 2mM, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
