3M7N

archaeoglobus fulgidus exosome with RNA bound to the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 43 2 2
Unit cell lengths	138.268, 138.268, 262.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.057 - 2.400
R-factor	0.2
Rwork	0.197
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ba1
RMSD bond length	0.008
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_129))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	94417
Completeness [%]	94.5	86.4
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M Na Acetate, pH 4.6, 30% MPD, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K