3M7M
Crystal structure of monomeric hsp33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-11 |
Detector | MAC Science DIP-2030 |
Wavelength(s) | 1. |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.981, 65.981, 145.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.060 - 2.900 |
R-factor | 0.22673 |
Rwork | 0.224 |
R-free | 0.28059 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7f |
RMSD bond length | 0.018 |
RMSD bond angle | 2.850 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.066 |
Number of reflections | 6752 |
<I/σ(I)> | 11.8 |
Completeness [%] | 91.5 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | ammonium sulfate, lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |