3M7J
Crystal structure of the bacteriocin LLPA from pseudomonas sp. in complex with Met-mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8073 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.973, 153.201, 33.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.644 - 2.260 |
| R-factor | 0.1778 |
| Rwork | 0.175 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m7h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.837 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.645 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.124 | 0.542 |
| Number of reflections | 33688 | |
| <I/σ(I)> | 7.1 | 2.6 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M imidazole, 1.3M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
