3M7H
Crystal structure of the bacteriocin LLPA from Pseudomonas sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2008-05-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8073 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 150.495, 154.492, 34.218 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.979 - 2.200 |
R-factor | 0.175 |
Rwork | 0.172 |
R-free | 0.20430 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.049 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.064 | 0.582 |
Number of reflections | 41385 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 100.0 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M imidazole, 1.3M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |