3M79
A tetrameric Zn-bound cytochrome cb562 complex with covalently and non-covalently stabilized interfaces crystallized in the presence of Cu(II) and Zn(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.547, 78.068, 178.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.870 - 2.100 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iq6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.095 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.170 | 2.210 |
High resolution limit [Å] | 2.099 | 2.100 |
Number of reflections | 49272 | |
<I/σ(I)> | 4 | 1.5 |
Completeness [%] | 93.6 | 84.2 |
Redundancy | 9.5 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |