3M79

A tetrameric Zn-bound cytochrome cb562 complex with covalently and non-covalently stabilized interfaces crystallized in the presence of Cu(II) and Zn(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.547, 78.068, 178.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.870 - 2.100
R-factor	0.225
Rwork	0.222
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iq6
RMSD bond length	0.009
RMSD bond angle	1.095
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.170	2.210
High resolution limit [Å]	2.099	2.100
Number of reflections	49272
<I/σ(I)>	4	1.5
Completeness [%]	93.6	84.2
Redundancy	9.5	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1