3M6X
Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus in space group P21212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-24 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.786, 109.093, 50.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.569 - 1.676 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m6u |
RMSD bond length | 0.004 |
RMSD bond angle | 0.936 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.740 |
High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
Rmerge | 0.150 | 0.099 | 0.487 |
Number of reflections | 110000 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.6 | 99.9 | 97.8 |
Redundancy | 6.4 | 7.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 8.5 | 277 | 160 mM Magnesium chloride hexahydrate, 80 mM TRIS-HCl (pH 8.5) and 24 % w/v PEG4000, microbatch under oil, temperature 277K |