3M6K
Crystal Structure of N-terminal 44 kDa fragment of topoisomerase V in the presence of guanidium hydrochloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2008-04-23 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.090, 70.090, 349.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.140 - 2.600 |
R-factor | 0.24371 |
Rwork | 0.241 |
R-free | 0.28883 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.203 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.079 | 0.572 |
Number of reflections | 28151 | |
<I/σ(I)> | 19 | 3.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.1 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 0.1 M phosphate-citrate pH 5, 0.2 M NaCl, 16% PEG 8000, 1M Guanidium hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 0.1 M phosphate-citrate pH 5, 0.2 M NaCl, 16% PEG 8000, 1M Guanidium hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 303K |