3M6G
Crystal structure of actin in complex with lobophorolide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.932, 86.419, 100.868 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.424 |
| Number of reflections | 60849 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 97.9 | 95.3 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1M MES pH 6.0, 6% methyl ether poly(ethylene glycol) 5000, 0.1M CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
