3M5W
Crystal Structure of Tryptophanyl-tRNA Synthetase from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.153, 50.604, 108.640 |
Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
Resolution | 47.811 - 2.320 |
R-factor | 0.187 |
Rwork | 0.183 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.462 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.320 | 2.320 |
Number of reflections | 29722 | |
<I/σ(I)> | 9.6 | 2.3 |
Completeness [%] | 99.6 | 97.3 |
Redundancy | 5.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 25 % w/v Polyehtlyene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |