3M5B
Crystal structure of the BTB domain from FAZF/ZBTB32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 49.076, 32.809, 69.425 |
Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
Resolution | 26.830 - 2.000 |
R-factor | 0.18916 |
Rwork | 0.186 |
R-free | 0.24388 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STARTING MODEL: SINGLE CHAIN POLY-ALANINE MODEL OF PDB CODE 1BUO EXCEPT LOOP REGIONS AND TURNS |
RMSD bond length | 0.023 |
RMSD bond angle | 1.792 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14434 | |
<I/σ(I)> | 16.95 | 3.77 |
Completeness [%] | 99.2 | 96.8 |
Redundancy | 2.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M ammonium sulfate, 0.35 M sodium citrate pH 5.5 and 30% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |