3M4Y
Structural characterization of the subunit A mutant P235A of the A-ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.564, 127.564, 105.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.930 - 2.380 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vdz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.440 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.040 | 0.342 |
Number of reflections | 35394 | |
<I/σ(I)> | 17.7 | 5.76 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 35% (v/v) MPD, 0.1M acetate (pH 4.5), vapor diffusion, hanging drop, temperature 291K |