3M4T
Crystal structure of the BTB domain from Kaiso/ZBTB33, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-06 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 117.208, 117.208, 38.405 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.300 - 2.050 |
| R-factor | 0.19314 |
| Rwork | 0.191 |
| R-free | 0.24181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Single chain poly-alanine model of PDB code 1BUO except where residues were identical between Kaiso and 1BUO and all loops and turns were deleted |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.532 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 10103 | |
| <I/σ(I)> | 51.82 | 8.43 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 20.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 295 | 0.25 M sodium sulfate, 0.1 M sodium acetate pH 4.3, 13% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
