3M3G
Crystal structure of Sm1, an elicitor of plant defence responses from Trichoderma virens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91959 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.238, 46.365, 88.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.030 - 1.390 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.20810 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.087 | 0.807 |
| Number of reflections | 40767 | |
| <I/σ(I)> | 99.6 | 9.4 |
| Redundancy | 6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 20% PEG 3350, 15mM ZnSO4, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
