3M2T
The crystal structure of dehydrogenase from Chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-02-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 138.860, 63.386, 103.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.814 - 2.300 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.23050 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.635 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.050 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.107 | 0.538 |
Number of reflections | 41479 | |
<I/σ(I)> | 19.8 | 5.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Sodium chloride, 0.1 M Bis-Tris pH 6.5, 1.5 M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |