3M2P
The crystal structure of UDP-N-acetylglucosamine 4-epimerase from Bacillus cereus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-02-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.116, 149.725, 166.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.701 - 2.950 |
R-factor | 0.2149 |
Rwork | 0.212 |
R-free | 0.27570 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.055 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.280 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.145 | 0.475 |
Number of reflections | 55533 | |
<I/σ(I)> | 13.5 | 5.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.3 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.4 M Sodium malonate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |