3M2K
Crystal Structure of fluorescein-labeled Class A -beta lactamase PenP in complex with cefotaxime
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Collection date | 2007-11-03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.538, 91.524, 66.302 |
| Unit cell angles | 90.00, 104.22, 90.00 |
Refinement procedure
| Resolution | 40.230 - 3.500 |
| R-factor | 0.17952 |
| Rwork | 0.176 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blm |
| RMSD bond length | 0.051 |
| RMSD bond angle | 3.999 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.148 |
| Number of reflections | 18395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | PEG 4000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
