3M2J
Crystal Structure of fluorescein-labeled Class A -lactamase PenP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Collection date | 2007-11-03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.354, 92.340, 66.415 |
| Unit cell angles | 90.00, 104.84, 90.00 |
Refinement procedure
| Resolution | 31.590 - 1.800 |
| R-factor | 0.21221 |
| Rwork | 0.209 |
| R-free | 0.27278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blm |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.220 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.084 |
| Number of reflections | 45125 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | PEG 4000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
