3M1M
Crystal structure of the primase-polymerase from Sulfolobus islandicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.718, 46.557, 77.993 |
| Unit cell angles | 90.00, 124.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.20644 |
| Rwork | 0.204 |
| R-free | 0.25074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ro2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.698 |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Total number of observations | 537575 |
| Number of reflections | 30362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 0.1M Tris-HCl pH 8.5, 30-35% PEG 4000, 200mM lithium sulphate, 2mM Tris-2-carboxyethyl-phosphine, VAPOR DIFFUSION, HANGING DROP |
