3M1C
Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 88.255, 88.255, 333.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.200 - 3.000 |
R-factor | 0.176 |
Rwork | 0.170 |
R-free | 0.24200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.215 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX (1.4_175) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.890 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.133 | |
Number of reflections | 32326 | |
<I/σ(I)> | 5.6 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 298 | 20% PEG 4000, 0.1M Sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |