3M18
Crystal structure of variable lymphocyte receptor VLRA.R2.1 in complex with hen egg lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.794, 68.116, 59.751 |
| Unit cell angles | 90.00, 110.94, 90.00 |
Refinement procedure
| Resolution | 55.810 - 1.950 |
| R-factor | 0.18425 |
| Rwork | 0.181 |
| R-free | 0.23989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3E6J PDB ENTRY 2VB1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.815 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.087 | 0.340 |
| Number of reflections | 24789 | |
| <I/σ(I)> | 33.2 | 3.2 |
| Completeness [%] | 80.2 | |
| Redundancy | 6.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% (w/v) polyethylene glycol 2000 and 0.1 M Bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
