3M0P
Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 4.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-04 |
Detector | CCD ADSC_Q210 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.918, 42.082, 48.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.600 |
R-factor | 0.205 |
Rwork | 0.201 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shg |
RMSD bond length | 0.012 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.799 | 2.690 |
High resolution limit [Å] | 2.593 | 2.600 |
Rmerge | 0.151 | 0.344 |
Number of reflections | 2201 | |
<I/σ(I)> | 8.6 | 5.2 |
Completeness [%] | 97.9 | 100 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.8 M ammonium sulphate, 0.1 M sodium acetate pH 4, vapor diffusion, hanging drop, temperature 298K |