3M0D
Crystal structure of the TRAF1:TRAF2:cIAP2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 41 |
Unit cell lengths | 91.867, 91.867, 88.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.500 - 2.800 |
R-factor | 0.204 |
Rwork | 0.197 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3M0A (TRAF2:ciap2) |
RMSD bond length | 0.011 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.097 | 0.044 | 0.478 |
Number of reflections | 9137 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.8 | 99.8 | 97.8 |
Redundancy | 6.1 | 6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.15M AmSO4, 0.1M MES, 15% PEG 4000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |