3M06
Crystal Structure of TRAF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | H 3 |
Unit cell lengths | 150.706, 150.706, 86.728 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.130 - 2.670 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m0a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.236 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.640 | 7.050 | 2.640 |
Rmerge | 0.068 | 0.040 | 0.376 |
Number of reflections | 20824 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 92.2 | 99.4 | 45.5 |
Redundancy | 5.8 | 5.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8% PEG3350, 6% Tacsimate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |