3LZX
Crystal structure of ferredoxin-NADP+ oxidoreductase from Bacillus subtilis (FORM II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.466, 64.850, 61.116 |
| Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
| Resolution | 26.850 - 1.900 |
| R-factor | 0.18267 |
| Rwork | 0.181 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.850 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.220 |
| Number of reflections | 59771 | |
| Completeness [%] | 96.9 | 80.4 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 3350, 0.2M Sodium fluoride, 5% Trehalose, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
