3LZN

Crystal Structure Analysis of the apo P19 protein from Campylobacter jejuni at 1.59 A at pH 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.282
Spacegroup name	P 21 21 2
Unit cell lengths	54.396, 73.037, 74.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.380 - 1.590
R-factor	0.14366
Rwork	0.142
R-free	0.18413
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P6222 INCOMPLETE SEMAD MODEL AT 2.8 ANGS RESOLUTION.
RMSD bond length	0.014
RMSD bond angle	1.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.110	1.680
High resolution limit [Å]	1.590	1.590
Rmerge	0.037	0.655
Number of reflections	40227
<I/σ(I)>	16.6	2.2
Completeness [%]	98.9	98.6
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	PROTEIN TREATED WITH 30X EXCESS OF EDTA; WASHED, PRECIPITANT WAS 25-45% POLYETHYLENE GLYCOL (PEG) 350, 0.1 M CHES (2-(N-CYCLOHEXYLAMINO) ETHANE SULFONIC ACID) BUFFER PH 9.0, CRYO FROZEN WITHOUT ANY ADDITION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K