3LZL
Crystal Structure Analysis of the as-solated P19 protein from Campylobacter jejuni at 1.45 A at pH 9.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-05 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.127 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.533, 73.545, 75.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.770 - 1.450 |
R-factor | 0.14337 |
Rwork | 0.141 |
R-free | 0.18668 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P6222 incomplete SeMAD model at 2.8 A resolution. |
RMSD bond length | 0.017 |
RMSD bond angle | 1.531 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.210 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.040 | 0.517 |
Number of reflections | 53633 | |
<I/σ(I)> | 14.8 | 2.3 |
Completeness [%] | 98.6 | 97.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 50% POLYETHYLENE GLYCOL (PEG) 250, 0.1 M CHES (2-(N-CYCLOHEXYLAMINO) ETHANE SULFONIC ACID) BUFFER pH 9.0, CRYOGENIC CONDITION 30% GLYCEROL IN PRECIPITANT MIXTURE, VAPOR DIFFUSION, HANGING DROP, temperature 298K |