3LZG
Crystal structure of a 2009 H1N1 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.677, 118.313, 120.073 |
| Unit cell angles | 117.37, 92.67, 100.84 |
Refinement procedure
| Resolution | 45.000 - 2.600 |
| R-factor | 0.1844 |
| Rwork | 0.181 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rd8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | |
| Number of reflections | 93210 | |
| <I/σ(I)> | 9.5 | 1.8 |
| Completeness [%] | 91.0 | 69.7 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 295.5 | 27% PEG-MME 2000, 0.1M Tris pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
